Phosphorylation of Acid-soluble Nucleolar Proteins of Novikoff Hepatoma Ascites Cells in Vivo
نویسندگان
چکیده
منابع مشابه
Phosphorylation of acid-soluble proteins in isolated nucleoli of Novikoff hepatoma ascites cells. Effects of divalent cations.
Isolated nucleoli from rat Novikoff hepatoma ascites cells incorporated 32P of [T-~~P]ATP into acid-soluble nucleolar proteins in vitro in systems containing 0.25 M sucrose, 5 mM MgCL, 12.5 mM NaCl, and 0.05 M Tris-HCl buffer (pH 7.3) at 37”. Although the total uptake of 32P reached a maximum after 15 min of incubation, the uptake into some protein bands increased with longer incubation times. ...
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Among the low molecular weight RNAs which have been shown to be localized to the nucleolus are the 4 S, U3, and 8 S RNAs. Of these, the U3 and 8 S RNAs are hydrogen bonded to nucleolar 28 S RNA. The nucleolar U3 RNA migrates as an electrophoretically homogeneous band on 8% polyacylamide gels but was separated into four bands on 16% gels. Although these bands have similar nucleotide compositions...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42704-x